General Cell Lines and Cell Cultures
Discovery Life Sciences Prostate Cancer Mutation PBMC
PBMCs Peripheral Blood Mononuclear Cells are immune cells isolated from whole blood and viably cryopreserved providing a foundational material for immunology studies cell therapy research and functional immune assays
Eurofins Discoverx ChemiScreen Ghrelin Receptor Stable Cell Line, 2 vials. (HTS187C)
The ChemiScreen Ghrelin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. ghrelin) based activation of GHSR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GHSR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of GHSR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
ATCC Transmissible; gastroenteritis virus; Strain: Purdue (attenuated)
Transmissible gastroenteritis virus strain Purdue (attenuated) is propagated in ST cells (ATCC CRL-1746). This strain was originally isolated from the small intestine of an infected pig with mild diarrhea and was deposited under the catalog number ATCC VR-763. It was then passaged through a 0.2 μM cellulose acetate membrane syringe filter and re-assigned to the catalog number ATCC VR-1995. This viral strain can be used in viral neutralization tests, agricultural research, and infectious and enteric disease research.
Eurofins Discoverx cAMP Hunter CHO-K1 CCR5 Gi Cell Line, 2 vials. (95-0068C2)
The cAMP Hunter CCR5 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL3) based activation of CCR5 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CCR5, a naturally Gi/Go coupled GPCR. Upon activation of CCR5, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx cAMP Hunter CHO-K1 CMKLR1 Gi Cell Line, 2 vials. (95-0080C2)
The cAMP Hunter CMKLR1 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. chemerin) based activation of CMKLR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CMKLR1, a naturally Gi/Go coupled GPCR. Upon activation of CMKLR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx Ready-to-Assay GPR109A Nicotinic Acid Receptor Frozen Cells, 2 Vials of Cells. (HTS201RTA)
The Ready-to-Assay GPR109A Nicotinic Acid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. nicotinic acid) based activation of HCAR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HCAR2 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of HCAR2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Medchemexpress LLC CD47 protein, rat (HEK293, Fc) | 95.0% | 500 UG
Recombinant rat CD47 extracellular domain expressed in HEK293 cells and supplied as a C-terminal human Fc-tagged fusion protein. Provided as a purified reagent for biochemical and cell-based research, it is suitable for binding studies, ELISA capture/detection, and receptor-ligand interaction assays. Supplied frozen; estimated purity ~95%.
- Recombinant rat CD47 extracellular domain with C-terminal human Fc tag.
- Expressed in HEK293 cells for mammalian-type glycosylation.
- Estimated purity 95.0%.
- Stored frozen at -20°C.
- Suitable for binding assays, ELISA capture/detection, and receptor interaction studies.
- Available in microgram-scale quantities (e.g., 500 UG).
Eurofins Discoverx KILR BT-474 Cell Pool, 2 vials. (97-1042P049)
The KILR BT-474 cell pool is a stable, engineered target cell pool, which closely reflects the heterogeneous native protein expression of tumor environments, used to measure direct cell death mediated by immunooncology treatments (e.g. checkpoint modulators, bispecifics, CAR-T, etc.) via Antibody Dependent Cell-mediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity (CDC), and Antibody Dependent Cell Phagocytosis (ADCP). This product uses EFC technology. The cell pool is a heterogeneous population of BT-474 cells that overexpress a ProLabel-tagged housekeeping gene. Upon effector cells-mediated cell lysis, ProLabel is released to the media, where EA in the detection kit produces an EFC signal proportional to the level of ProLabel released. This allows for calculation of percent lysis of the KILR cell pool. This stable cell pool is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kilr.
Biochain Institute Inc Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Stomach, 0.2 x2 mg /PK
BioChain's human total protein matched pair products include: Primary Pair (PP), Primary and Metastatic Pair (PM). PP consists of total protein isolated from primary tumor and its adjacent normal tissue; PM consists of total protein from primary tumor andcorresponding metastastic tumor. Total proteins in each pair are prepared from the same donor. This product line is designedfor identifying tumor-specific genes and tumor metastasis genes.
Eurofins Discoverx PathHunter U2OS TrkA Functional Assay, 2 vials. (93-0462C3)
The PathHunter U2OS TrkA Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkA dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkA and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter U2OS TORC2 Nuclear Translocation Cell Line, 2 vials. (93-0813C3)
The PathHunter U2OS TORC2 Nuclear Translocation Cell Line is a stable, engineered cell line for use in studying drug candidates affecting the CRTC/CREB pathway. This cell line enables assessment of ligand (e.g. forskolin) based pathway activation via detection of resulting TORC2 (CRTC2) nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged TORC2 (CRTC2) and nuclear localized EA. Upon pathway activation, induced nuclear translocation of TORC2 leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/pathways.
Medchemexpress LLC CD47 protein, rat (HEK293, His) | >95.0% | 10 UG
Recombinant rat CD47 extracellular domain expressed in HEK293 cells with a C-terminal His tag. Supplied lyophilized and intended for research use; the protein is highly pure and shows heterogeneous migration by SDS-PAGE due to glycosylation.
- Recombinant extracellular domain (Q19-K140) with C-terminal His tag.
- High purity (>95%) as determined by reducing SDS-PAGE.
- Apparent molecular weight 23-40 kDa because of glycosylation.
- Lyophilized formulation from 0.22 μm filtered PBS, pH 7.4.
- Recommended reconstitution at ≥100 μg/mL in ddH2O; aliquot and store at -20°C or -80°C for long term.
- Suitable for immunoassays, binding studies, and antibody characterization.
Eurofins Discoverx Ready-to-Assay κ Opioid Receptor Frozen Cells, 2 Vials of Cells. (HTS095RTA)
The Ready-to-Assay κ Opioid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. DADLE, DPDPE) based activation of OPRK1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses OPRK1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of OPRK1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Medchemexpress LLC GIP Human HEK293 1mg
The GIP protein is a member of the glucagon superfamily an important incretin hormone that stimulates pancreatic beta cells to secrete insulin in response to food intake Through its G protein-coupled receptor it activates adenylyl cyclase and other signal transduction pathways GIP Protein Human (HEK293 hFc) is the recombinant human-derived GIP protein expressed by HEK293 with C-hFc labeled tag
Eurofins Discoverx PathHunter CHO-K1 mCXCR5 β-Arrestin Cell Line, 2 vials. (93-0474C2)
The PathHunter mCXCR5 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CXCL13/BCA-1/BLC) based activation of mCXCR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCXCR5 and EA-tagged β-Arrestin-2. Activation of mCXCR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.